Table 2 Primers of RT-PCR, ChIP and coding sequences cloning
From: STAT4 targets KISS1 to promote the apoptosis of ovarian granulosa cells
| Name | Sequence | Product (bp) | Accession number |
|---|---|---|---|
| CDS-STAT4 | F: GGGGTACCATGTCTCAGTGGAATCAAGTC | 2266 | NM_001197305.1 |
| R: CCAAGCTTTCAGTCTGAGTCAGGTCCTT | |||
| CDS-KISS1 | F: CCGAATTCATGAATGCACTGGTTTTTTGG | 431 | NM_001134964.1 |
| R: GGCGCCGGCGAGTCAGAGCGGGCCGCGGAA | |||
| qRT-PCR-KISS1 | F: AACCAGCATCTTCTCACCAGG | 192 | NM_001134964.1 |
| R: CTTTCTCTCCGCACAACGC | |||
| qRT-PCR-STAT4 | F: TTGTCTGCTCTACCATTCGCTG | 182 | NM_001197305.1 |
| R: TAACCTTTGTCTCCCCTTTCTG | |||
| qRT-PCR-GAPDH | F: TCCCGCCAACATCAAAT | 163 | XM_021091114.1 |
| R: CACGCCCATCACAAACAT | |||
| qRT-PCR-PIK3CG | F: AACGGGCTTTGAGATAGTGAA | 184 | NM_213939.1 |
| R: AAGTTGCTTGGTTGGTGGATA | |||
| qRT-PCR-PIK3C1 | F: CAAGTGAGAATGGTCCGAATG | 152 | NM_006218.3 |
| R: GTGGAAGAGTTTGCCTGTTTT | |||
| qRT-PCR-PDK1 | F: AAATCACCAGGACAGCCAATA | 190 | NM_001159608.1 |
| R: CTTCTCGGTCACTCATCTTCAC | |||
| qRT-PCR-FOXO3 | F: ACAAACGGCTCACTCTGTCCCA | 85 | NM_001135959.1 |
| R: GAACTGTTGCTGTCGCCCTTATC | |||
| qRT-PCR-TSC2 | F: CGAGGTGGTGTCCTACGAGAT | 115 | XM_005655162.3 |
| R: GAGCAGGCGTTCAATGATGTT | |||
| qRT-PCR-BAD | F: AGTCGCCACTGCTCTTACCC | 172 | XM_021082883.1 |
| R: TCTTGAAGGAACCCTGGAAATC | |||
| qRT-PCR-Star | F: GGAAAAGACACAGTCATCACCCAT | 121 | NM_213755.2 |
| R: CAGCAAGCACACACACGGAAC | |||
| qRT-PCR-CYP17 | F: AAGCCAAGACGAACGCAGAAAG | 228 | NM_214428.1 |
| R: TAGATGGGGCACGATTGAAACC | |||
| qRT-PCR-3B-HSD | F: GGGGCTTCTGTCTTGATTCCA | 284 | NM_001004049.2 |
| R: GGTTTTCAGTGCTTCCTTGTGC | |||
| qRT-PCR-17B-HSD | F: CCCAACGCAGGAGACTCAAAAT | 149 | NM_214306.1 |
| R: CCAGAGCCCATAACGAAGACAGA | |||
| qRT-PCR-CYP19A | F: GCTGGACACCTCTAACAACCTCTT | 91 | NM_214430.1 |
| R: TTGCCATTCATCAAAATAACCCT | |||
| qRT-PCR-ESR1 | F: GATGCCTTGGTCTGGGTGAT | 124 | XM_003468423.2 |
| R: AGTGTTCCGTGCCCTTGTTA | |||
| qRT-PCR-ESR2 | F: AAGGGAAAAGGAGGATGGGACA | 202 | NM_0010011533.1 |
| R: CAGATAGGGACTGCGTGGAGGT | |||
| ChIP-STAT4 | F: CCTTGCCCACTTCACTCCAC | 153 | NM_001197305.1 |
| R: AGGACAGAAGGAATCGAGGGA | |||
| ChIP-GAPDH | F: GATGTCCTGAGCCCCTACAG | 102 | XM_021091114.1 |
| R: GGTAGGTGATGGGGACTGAG |